What can AB do for you?
Applied BioAnalytics is an independent, privately owned CRO / analytical testing and method development service provider, specialized in the academic and early phase industrial biologics and biosimilar R&D pipelines. Our approach to innovation in CE leads to not just increased efficiencies, but also improved data quality. Additionally, we are competitively priced due to our location in Hungary.
In a nutshell, we are:
▪ A biomolecular characterization company
▪ Built on a legacy of capillary electrophoresis (CE) expertise
▪ Founded in 2020 by an experienced, multilingual management team
▪ with strong academic background in bioanalytical chemistry
▪ 20+ years academic and industrial experience in the field
▪ Having tight links and access to academic and pharmaceutical laboratory resources
▪ Active strong collaborations with thought leaders in the field
▪ Competitively priced due to location in Hungary
We look forward to serve you with analysis of samples, method development & qualification, a flat baseline CE-SDS gel for simple peak integration, and SDS-CAGE-MS.
Research & Insights
Publications
B. Meszaros, et al; Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis, Journal of Chromatography B, 1137 (2020) 121913. https://pubmed.ncbi.nlm.nih.gov/31881512/
Szekrenyes, et al; Quantitative comparison of mAb N-glycosylation using the Glycosimilarity Index, A Tutorial, TrAC, 122 (2020) 115728. https://www.sciencedirect.com/science/article/pii/S0165993619305849
M. Szigeti, et al; Sample preparation scale-up for deep N-glycomic analysis of human serum by capillary electrophoresis and CE-ESI-MS, Molecular and Cellular Proteomics, 18 (2019) 2524-2531. https://pubmed.ncbi.nlm.nih.gov/31628258/
Zs. Elek, et al; High throughput multiplex SNP-analysis in chronic obstructive pulmonary disease and lung cancer, Current Molecular Medicine, 20 (2020) 185-193. https://pubmed.ncbi.nlm.nih.gov/31644407/
M. L. A. De Leoz, et al; NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods, Molecular and Cellular Proteomics, 19 (2020) 11-30. https://pubmed.ncbi.nlm.nih.gov/31591262/
G. Jarvas, et al; Expanding the capillary electrophoresis based glucose unit database of the GUcal app, Glycobiology, In Press. https://pubmed.ncbi.nlm.nih.gov/31829415/
B. Donczo, et al; Effect of the elapsed time between sampling and formalin fixation on the N-glycosylation profile of mouse tissue specimens, Electrophoresis, 40 (2019) 3057–3061. https://pubmed.ncbi.nlm.nih.gov/31535724/
Cs. Filep, et al; N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: generation and application of a new glucose unit database, Journal of Pharmaceutical and Biomedical Analysis, 178 (2020) 112892. https://pubmed.ncbi.nlm.nih.gov/31593866/
M. Szarka, et al; Imaging laser induced fluorescence detection at the Taylor cone of electrospray ionization mass spectrometry, Analytical Chemistry, 91 (2019) 7738-7743. https://pubmed.ncbi.nlm.nih.gov/31136147/
M. Szabo, et al; Proteomic and Glycomic Markers to Differentiate Lung Adenocarcinoma from COPD, Current Medicinal Chemistry, (2019) In Press. https://pubmed.ncbi.nlm.nih.gov/30332938/
B. Reider, et al; Evaporative fluorophore labeling of carbohydrates via reductive amination, Talanta, 185 (2018) 365-369. https://pubmed.ncbi.nlm.nih.gov/29759213/
B. Donczo, et al; Biomedical analysis of formalin-fixed, paraffin-embedded tissue samples: The Holy Grail for molecular diagnostics, Journal of Pharmaceutical and Biomedical Analysis, 155 (2018) 125-134. https://pubmed.ncbi.nlm.nih.gov/29627729/
M. Szigeti, et al; Quantitative assessment of mAb Fc glycosylation of CQA importance by capillary electrophoresis, Electrophoresis, 39 (2018) 2340-2343. https://pubmed.ncbi.nlm.nih.gov/29600574/
T. G. Szilágyi, et al; Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection, Food Chemistry, 256 (2018) 129-132. https://pubmed.ncbi.nlm.nih.gov/29606428/
G. Jarvas, et al; Effect of the flow profile on separation efficiency in pressure‐assisted reversed‐polarity capillary zone electrophoresis of anions: Simulation and experimental evaluation, Journal of Separation Science, 41 (2018) 2473-2478. https://pubmed.ncbi.nlm.nih.gov/29457870/
B. Borza, et al; Glycosimilarity assessment of biotherapeutics 1: Quantitative comparison of the N-glycosylation of the innovator and a biosimilar version of etanercept, Journal of Pharmaceutical and Biomedical Analysis, 153 (2018) 182-185. https://pubmed.ncbi.nlm.nih.gov/29499461/
A. Szekrenyes, et al; Multi-Site N-Glycan Mapping Study 2: UHPLC, Electrophoresis, 39 (2018) 998-1005. https://pubmed.ncbi.nlm.nih.gov/29330871/
B. Meszaros, et al; Quantitative characterization of plasma treated PDMS microfluidic substrates by inverse gas chromatography, Sensors and Actuators B, 258 (2018) 1184-1190. https://www.sciencedirect.com/science/article/pii/S092540051732316X
D. Sarkozy, et al; Capillary electrophoresis analysis of industrial galactooligosaccharides, J Pharm Biomed Anal, 233 (2023) 115434. https://pubmed.ncbi.nlm.nih.gov/37196591/
D. Sarkozy, et al; Activation energy of SDS-proteins in capillary electrophoresis with tetrahydroxyborate cross-linked agarose gels, Gels, In Press (2024) (HLBS 216).
R. Farsang et al; Purification-free N-glycan analysis by capillary zone electrophoresis: Hunt for the lost glycans, Journal of Pharmaceutical and Biomedical Analysis, 238 (2024) 115812. https://pubmed.ncbi.nlm.nih.gov/37926036/
L. Hajba et al; Ionic liquids in capillary electrophoresis analysis of proteins and carbohydrates, Journal of Chromatography A, 1716 (2024) 464642. https://pubmed.ncbi.nlm.nih.gov/38237290/
R. Farsang et al; Glucose Unit Computation in Capillary Zone Electrophoresis of Carbohydrates using a Numerical Approximation Based Search for a Virtual EOF Marker. A Tutorial, TrAC, 170 (2024) 117762. https://www.sciencedirect.com/science/article/pii/S0165993624002450
F. Auer et al; In-Migration Noncovalent Fluorophore Labeling of Proteins by Propidium Iodide in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis, Analytical Chemistry, 96 (2024) 10969-10977. https://pubmed.ncbi.nlm.nih.gov/38938066/
J. Šesták et al; Fluorescence detection setups in capillary scale electrophoresis and microscale liquid chromatography: Developments in the past 15 years, TrAC, 181 (2024) 118001. https://www.sciencedirect.com/science/article/abs/pii/S0165993624004849
D. Sarkozy et al; Activation energy of SDS-proteins in capillary electrophoresis with tetrahydroxyborate cross-linked agarose gels, Gels, In Press (2024).
Presentations
Deep N-glycomic analysis of human serum by capillary electrophoresis and CESI-MS: the challenge of sample preparation, LACE-2019, Alcala, Spain, Sept 29-30, 2019.
Multicapillary gel electrophoresis analysis of N-glycans, CECE-2019, Gdansk, Poland, Sept 24-26, 2019.
Comprehensive N-glycosylation characterization of therapeutic antibodies by capillary electrophoresis and mass spectrometry EuroCARB 2019, Leiden, The Netherlands, Jun 30-July 4, 2019.
LC or CE? Maybe both! Towards comprehensive characterization of N-glycosylation modifications of protein biopharmaceuticals, HPLC 2019, June 16-20, 2019 Milan, IT
Comparative Glycosylation Analysis of Biotherapeutics and their Biosimilars, Pittcon, 2019, Philadelphia, PA, March 17-21, 2019.
Validation of a fast and high resolution capillary electrophoresis based mAb N-glycan profiling method, AT Europe 2019, Dublin, Ireland, 12-15 March, 2019.
Fully automated sequencing of complex carbohydrates, LACE 2018, Mendoza, AR, Dec 2-4, 2018.
Posters
N-glycosylation analysis of therapeutic glycoproteins by multicapillary gel electrophoresis, PBA-019, Tel Aviv, IL, Sept 15-18, 2019.
Analysis of fluorophore labeled N-glycans using liquid phase separation methods, HPLC 2019, Milan, Italy, June 16-20, 2019.
Fast glycan sequencing using a fully automated carbohydrate sequencer, LACE-2018, Mendoza, Argentina, Dec 1-4, 2018.
Comparative characterization of the Fc domain N-glycosylation in monoclonal antibody and fusion protein therapeutics by CGE-LIF and UHPLC-FL, LACE-2018, Mendoza, Argentina, Dec 1-4, 2018.
High efficiency evaporative fluorophore labeling of glycans, CECE 2018, Brno, Czech Republic, Oct 15-17, 2018.
Publications
B. Meszaros, et al; Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis, Journal of Chromatography B, 1137 (2020) 121913. link
Szekrenyes, et al; Quantitative comparison of mAb N-glycosylation using the Glycosimilarity Index, A Tutorial, TrAC, 122 (2020) 115728. link
M. Szigeti, et al; Sample preparation scale-up for deep N-glycomic analysis of human serum by capillary electrophoresis and CE-ESI-MS, Molecular and Cellular Proteomics, 18 (2019) 2524-2531. link
Zs. Elek, et al; High throughput multiplex SNP-analysis in chronic obstructive pulmonary disease and lung cancer, Current Molecular Medicine, 20 (2020) 185-193. link
M. L. A. De Leoz, et al; NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods, Molecular and Cellular Proteomics, 19 (2020) 11-30. link
G. Jarvas, et al; Expanding the capillary electrophoresis based glucose unit database of the GUcal app, Glycobiology, In Press. link
B. Donczo, et al; Effect of the elapsed time between sampling and formalin fixation on the N-glycosylation profile of mouse tissue specimens, Electrophoresis, 40 (2019) 3057–3061. link
Cs. Filep, et al; N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: generation and application of a new glucose unit database, Journal of Pharmaceutical and Biomedical Analysis, 178 (2020) 112892. link
M. Szarka, et al; Imaging laser induced fluorescence detection at the Taylor cone of electrospray ionization mass spectrometry, Analytical Chemistry, 91 (2019) 7738-7743. link
M. Szabo, et al; Proteomic and Glycomic Markers to Differentiate Lung Adenocarcinoma from COPD, Current Medicinal Chemistry, (2019) In Press. link
B. Reider, et al; Evaporative fluorophore labeling of carbohydrates via reductive amination, Talanta, 185 (2018) 365-369. link
B. Donczo, et al; Biomedical analysis of formalin-fixed, paraffin-embedded tissue samples: The Holy Grail for molecular diagnostics, Journal of Pharmaceutical and Biomedical Analysis, 155 (2018) 125-134. link
M. Szigeti, et al; Quantitative assessment of mAb Fc glycosylation of CQA importance by capillary electrophoresis, Electrophoresis, 39 (2018) 2340-2343. link
T. G. Szilágyi, et al; Analysis of the oligosaccharide composition in wort samples by capillary electrophoresis with laser induced fluorescence detection, Food Chemistry, 256 (2018) 129-132. link
G. Jarvas, et al; Effect of the flow profile on separation efficiency in pressure‐assisted reversed‐polarity capillary zone electrophoresis of anions: Simulation and experimental evaluation, Journal of Separation Science, 41 (2018) 2473-2478. link
B. Borza, et al; Glycosimilarity assessment of biotherapeutics 1: Quantitative comparison of the N-glycosylation of the innovator and a biosimilar version of etanercept, Journal of Pharmaceutical and Biomedical Analysis, 153 (2018) 182-185. link
A. Szekrenyes, et al; Multi-Site N-Glycan Mapping Study 2: UHPLC, Electrophoresis, 39 (2018) 998-1005. link
B. Meszaros, et al; Quantitative characterization of plasma treated PDMS microfluidic substrates by inverse gas chromatography, Sensors and Actuators B, 258 (2018) 1184-1190. link
D. Sarkozy, et al; Capillary electrophoresis analysis of industrial galactooligosaccharides, J Pharm Biomed Anal, 233 (2023) 115434. link
D. Sarkozy, et al; Activation energy of SDS-proteins in capillary electrophoresis with tetrahydroxyborate cross-linked agarose gels, Gels, In Press (2024) (HLBS 216).
R. Farsang et al; Purification-free N-glycan analysis by capillary zone electrophoresis: Hunt for the lost glycans, Journal of Pharmaceutical and Biomedical Analysis, 238 (2024) 115812. link
L. Hajba et al; Ionic liquids in capillary electrophoresis analysis of proteins and carbohydrates, Journal of Chromatography A, 1716 (2024) 464642. link
R. Farsang et al; Glucose Unit Computation in Capillary Zone Electrophoresis of Carbohydrates using a Numerical Approximation Based Search for a Virtual EOF Marker. A Tutorial, TrAC, 170 (2024) 117762. link
F. Auer et al; In-Migration Noncovalent Fluorophore Labeling of Proteins by Propidium Iodide in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis, Analytical Chemistry, 96 (2024) 10969-10977. link
J. Šesták et al; Fluorescence detection setups in capillary scale electrophoresis and microscale liquid chromatography: Developments in the past 15 years, TrAC, 181 (2024) 118001. link
D. Sarkozy et al; Activation energy of SDS-proteins in capillary electrophoresis with tetrahydroxyborate cross-linked agarose gels, Gels, In Press (2024).
Presentations
Deep N-glycomic analysis of human serum by capillary electrophoresis and CESI-MS: the challenge of sample preparation, LACE-2019, Alcala, Spain, Sept 29-30, 2019.
Multicapillary gel electrophoresis analysis of N-glycans, CECE-2019, Gdansk, Poland, Sept 24-26, 2019.
Comprehensive N-glycosylation characterization of therapeutic antibodies by capillary electrophoresis and mass spectrometry EuroCARB 2019, Leiden, The Netherlands, Jun 30-July 4, 2019.
LC or CE? Maybe both! Towards comprehensive characterization of N-glycosylation modifications of protein biopharmaceuticals, HPLC 2019, June 16-20, 2019 Milan, IT
Comparative Glycosylation Analysis of Biotherapeutics and their Biosimilars, Pittcon, 2019, Philadelphia, PA, March 17-21, 2019.
Validation of a fast and high resolution capillary electrophoresis based mAb N-glycan profiling method, AT Europe 2019, Dublin, Ireland, 12-15 March, 2019.
Fully automated sequencing of complex carbohydrates, LACE 2018, Mendoza, AR, Dec 2-4, 2018.
Posters
N-glycosylation analysis of therapeutic glycoproteins by multicapillary gel electrophoresis, PBA-019, Tel Aviv, IL, Sept 15-18, 2019.
Analysis of fluorophore labeled N-glycans using liquid phase separation methods, HPLC 2019, Milan, Italy, June 16-20, 2019.
Fast glycan sequencing using a fully automated carbohydrate sequencer, LACE-2018, Mendoza, Argentina, Dec 1-4, 2018.
Comparative characterization of the Fc domain N-glycosylation in monoclonal antibody and fusion protein therapeutics by CGE-LIF and UHPLC-FL, LACE-2018, Mendoza, Argentina, Dec 1-4, 2018.
High efficiency evaporative fluorophore labeling of glycans, CECE 2018, Brno, Czech Republic, Oct 15-17, 2018.